anti α tubulin primary antibody (Cell Signaling Technology Inc)
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Anti α Tubulin Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 5406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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other:Article Title: Evidence of histidine and aspartic acid phosphorylation in human prostate cancer cells. Article Snippet: We have developed a method to identify previously undetected histidine and aspartic acid phosphorylations in a human p ros t a t e cance r p rog r e s s i on mode l . A phosphoproteome of our cell line model is presented, with correlation of modified protein expression between the three states of cancer: non-tumorigenic, tumorigenic, and metastatic cells.. With the described interaction proteins potentially phosphorylated by NM23-H1, cellular responses to motility and conformational change stimuli would be achievable.. We detect 20 novel histidine-phosphorylated (pHis) and 80 novel aspartic acid-phosphorylated (pAsp) proteins with diverse functions, such as metabolism, protein folding, and motility. Control:Article Title: A single cluster of RNA Polymerase II molecules is stably associated with active genes. Article Snippet: Proteins were immunodetected using mouse antiRPB1 CTD Pan primary antibody (1:10 dilution; Cell Signaling Technology, 2629 T), followed by HRP-conjugated anti-mouse secondary antibody and chemiluminescent detection using the Jess HRP Detection Reagent. .. For the loading control, equivalent amounts of protein from each genotype were analyzed using the 12–230 kDa Separation Module and detected with Article Title: A single cluster of RNA Polymerase II molecules is stably associated with active genes Article Snippet: Proteins were immunodetected using mouse anti- RPB1 CTD Pan primary antibody (1:10 dilution; Cell Signaling Technology, 2629 T), followed by HRP-conjugated anti-mouse secondary antibody and chemiluminescent detection using the Jess HRP Detection Reagent. .. For the loading control, equivalent amounts of protein from each genotype were analyzed using the 12–230 kDa Separation Module and detected with Staining:Article Title: Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46 Article Snippet: .. Microtubule staining was performed post-fixation using an |
![sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to <t>β-tubulin,</t> in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2195/pmc13062195/pmc13062195__fphar-17-1741914-g001.jpg)