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anti α tubulin primary antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti α tubulin primary antibody
    Anti α Tubulin Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 5406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+%CE%B1+tubulin+primary+antibody/alpha-Tubulin+Antibody/pm41862494-280-22-27
    Average 97 stars, based on 5406 article reviews
    anti α tubulin primary antibody - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    other:

    Article Title: Evidence of histidine and aspartic acid phosphorylation in human prostate cancer cells.
    Article Snippet: We have developed a method to identify previously undetected histidine and aspartic acid phosphorylations in a human p ros t a t e cance r p rog r e s s i on mode l . A phosphoproteome of our cell line model is presented, with correlation of modified protein expression between the three states of cancer: non-tumorigenic, tumorigenic, and metastatic cells.. With the described interaction proteins potentially phosphorylated by NM23-H1, cellular responses to motility and conformational change stimuli would be achievable.. We detect 20 novel histidine-phosphorylated (pHis) and 80 novel aspartic acid-phosphorylated (pAsp) proteins with diverse functions, such as metabolism, protein folding, and motility.

    Control:

    Article Title: A single cluster of RNA Polymerase II molecules is stably associated with active genes.
    Article Snippet: Proteins were immunodetected using mouse antiRPB1 CTD Pan primary antibody (1:10 dilution; Cell Signaling Technology, 2629 T), followed by HRP-conjugated anti-mouse secondary antibody and chemiluminescent detection using the Jess HRP Detection Reagent. .. For the loading control, equivalent amounts of protein from each genotype were analyzed using the 12–230 kDa Separation Module and detected with anti-α-tubulin primary antibody (1:10 dilution; Cell Signaling Technology, 2144), followed by HRP-conjugated secondary antibody and Jess HRP Detection Reagent. ..

    Article Title: A single cluster of RNA Polymerase II molecules is stably associated with active genes
    Article Snippet: Proteins were immunodetected using mouse anti- RPB1 CTD Pan primary antibody (1:10 dilution; Cell Signaling Technology, 2629 T), followed by HRP-conjugated anti-mouse secondary antibody and chemiluminescent detection using the Jess HRP Detection Reagent. .. For the loading control, equivalent amounts of protein from each genotype were analyzed using the 12–230 kDa Separation Module and detected with anti-α-tubulin primary antibody (1:10 dilution; Cell Signaling Technology, 2144), followed by HRP-conjugated secondary antibody and Jess HRP Detection Reagent. ..

    Staining:

    Article Title: Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46
    Article Snippet: .. Microtubule staining was performed post-fixation using an anti-α-tubulin primary antibody (1:200, CST, Danvers, MA, USA) and a DyLight 405-labeled anti-mouse secondary antibody (KPL, Gaithersburg, MD, USA). .. Trim46 was detected using a rabbit anti-Trim46 antibody (1:25, Proteintech, Chicago, IL, USA) and a Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor ® 555 conjugate (Thermo Fisher Scientific, Rockford, IL, USA).



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    sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to <t>β-tubulin,</t> in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.
    Hfab Rhodamine Anti Tubulin Primary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti α tubulin primary antibody
    sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to <t>β-tubulin,</t> in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.
    Anti α Tubulin Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech primary antibodies against α tubulin
    sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to <t>β-tubulin,</t> in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.
    Primary Antibodies Against α Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology primary antibodies
    sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to <t>β-tubulin,</t> in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.
    Primary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to β-tubulin, in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Soluble epoxide hydrolase inhibition restores pro-resolving lipid mediators and reduces inflammation in localized provoked vulvodynia

    doi: 10.3389/fphar.2026.1741914

    Figure Lengend Snippet: sEH is involved in the LPV inflammatory process. (A) Illustration of punch biopsy collection from LPV cases and healthy controls during surgery. Light touch to the vulvar vestibule (Vest) is immensely painful, whereas the adjacent external vulva (Vulv) is not painful to touch. (B) RT-qPCR data showing sEH (EPHX2) mRNA expression, normalized to 18S rRNA, in LPV and control fibroblasts (n = 6 cases and 6 controls). (C) Western blot data showing sEH (EPHX2) protein expression, normalized to β-tubulin, in LPV and control fibroblasts (n = 3 cases and 3 controls). (D) sEH enzyme activity is significantly elevated in the LPV vest compared to LPV vulv, control vest, and control vulv (n = 3 cases and 3 controls). The data was collected in technical triplicates and are represented as mean ± SEM, one-way ANOVA + Tukey’s post hoc test, *P < 0.05, ns = not significant. (E) siRNA knockdown with sEH siRNA [5 µg] in vulvar and vestibular fibroblasts derived from 3 LPV cases was confirmed through Western blotting. (F) Transfection of sEH siRNA in LPV case vestibular and vulvar fibroblasts, but not control siRNA, reduced IL-6 and PGE2 production upon IL-1β [500 pg/mL] stimulation (n = 3 cases). The data was collected in six technical replicates and are represented as mean ± SEM, two-way ANOVA + Tukey’s post hoc test, *P < 0.05.

    Article Snippet: Protein blots were probed with EPHX2 rabbit polyclonal antibody (Abclonal, Woburn, MA, Cat: A1885) at a dilution of 1:1,000, hFAB Rhodamine Anti-Tubulin Primary Antibody (Bio-Rad, Cat: #12004166) at a dilution of 1:5,000, and peroxidase AffiniPure goat anti-rabbit IgG (H + L) (Jackson ImmunoResearch, West Grove, PA, Cat: 111-035-003) at a dilution of 1:4,000.

    Techniques: Quantitative RT-PCR, Expressing, Control, Western Blot, Activity Assay, Knockdown, Derivative Assay, Transfection